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Ultra-high resolution structural and molecular imaging of cells and tissues

Friday November 18, 2022
3:30 pm

 Presenter:  Fang Huang, Purdue University
 Series:  Physics and Astronomy Colloquium
 Abstract:  We are in an exciting era of biomedical imaging where the inner-workings of cells and tissues can be explored by rapidly developing imaging methods. Labeling specificity and live cell compatibility make fluorescence microscopy an important tool in biomedical research. Its resolution, however, is limited by diffraction to ~250 nm, preventing us from resolving detailed structures within the cell. The recent advent of single molecule switching nanoscopy methods (SMSN, also known as PALM/STORM), overcomes this fundamental limit by stochastically switching single fluorophores on and off so that their emission events can be localized with high precision resulting in a reconstructed image with down to ~25 nm lateral resolution. However, its application has been largely limited to fixed and flat samples due to the poor temporal resolution, inferior resolution in z, and rapidly deteriorating resolution in thick samples.

In this talk, I will present some of our most recent developments which synergistically combine newly available sensors/devices such as sCMOS cameras and deformable mirrors, analytical methods such as deep learning and novel instrumentation to allow SMSN imaging in live cells and tissue specimens. I will show the capabilities of these new imaging systems in revealing the fine details of subcellular structures from a diverse set of biological systems including viruses, bacteria, yeasts, mammalian cells and brain sections.
 Host:  Keith Lidke
 Location:  PAIS-1100, PAIS

Disability Notice If you need an auxiliary aid or service to attend any Department of Physics and Astronomy event, please contact the department (phone: 505 277-2616; email: physics@unm.edu) as far in advance as possible to ensure you are accommodated.