Imaging Cellular Components and Dynamics with Sub-Diffraction Resolution
Thursday March 2, 2017
|Presenter:||Professor Keith Lidke, UNM, Physics and Astronomy|
Due to the near transparency of cells, optical imaging is well suited for studying live or intact cells. Tagging cellular components with fluorophores dramatically extends these capabilities by providing molecular specificity and improved contrast. However, the light microscope is limited by diffraction to ~ 300 nm, whereas many important events for cell function and signaling involve interactions of proteins that occur at the ~ 10 nm scale. I will discuss methods for circumventing the diffraction limit using single molecule fluorescence imaging with a focus on technical development and remaining issues.
|Location:||Room 103, Center for High Tech Materials|